i-scei rare-cutting endonuclease saccharomyces cerevisiae expression vector (pcbascei  (Addgene inc)

Journal:

Article Title: Improved and Versatile Transformation System Allowing Multiple Genetic Manipulations of the Hyperthermophilic Archaeon Thermococcus kodakaraensis

doi: 10.1128/AEM.71.7.3889-3899.2005

Figure Lengend Snippet: Strains and plasmids used in this study

Article Snippet: Further modifications of the medium for investigation of auxotrophy of mutant strains and selection of transformants are described in the text. table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Strain or plasmid Relevant characteristic(s) Source or reference Strains E. coli DH5α supE44 Δ lacU169 (Φ80 lacZ Δ M15 ) hsdR17 recA1 endA1 gyrA96 thi-1 relAI Stratagene (La Jolla, CA) T. kodakaraensis KOD1 Wild type 5 , 16 KU216 Δ pyrF This study KW128 KU216 Δ trpE :: pyrF This study KH3 KW128 Δ hisD :: trpE This study KuW1 KU216 Δ trpE ::3′ region of trpE-pyrF This study KUW1 Δ pyrF Δ trpE This study KuWH1 KUW1 Δ hisD ::3′ region of hisD-pyrF This study KUWH1 Δ pyrF Δ trpE Δ hisD This study KuWc1 KU216 Δ trpE ::2μ′= pyrF -2μ′ This study KUWc1 Δ pyrF Δ trpE ::2μ′ This study KuWcHc1 KUWc1 Δ hisD ::2μ′- pyrF -2μ′ This study KUWcHc1 Δ pyrF Δ trpE ::2μ′ Δ hisD ::2μ′ This study Plasmids pUC118 Amp r general cloning vector Takara Bio (Ohtsu, Japan) pUD2 pUC118 derivative, pyrF marker cassette (P pyrF :: pyrF ) This study pUMT2 pUC118 derivative; trpE marker cassette (P pyrF :: trpE ) 22 pUCMP pUC118 derivative; 2μ′- pyrF -2μ′ This study pUDPyrF pUC118 derivative; Δ pyrF This study pUDTrpE pUC118 derivative; Δ trpE :: pyrF This study pUDHisD pUC118 derivative; Δ hisD :: trpE This study pUDLysV pUC118 derivative; Δ lysV :: trpE This study pUDTPOP pUC118 derivative; Δ trpE ::3′ region of trpE-pyrF This study pUDHPOP pUC118 derivative; Δ hisD ::3′ region of hisD-pyrF This study pYES2 General expression vector for Saccharomyces cerevisiae Invitrogen (Carlsbad, CA) pUDTPOPC pUC118 derivative; Δ trpE ::2μ′- pyrF -2μ′ This study pUDHPOPC pUC118 derivative; Δ hisD ::2μ′- pyrF -2μ′ This study Open in a separate window Strains and plasmids used in this study Escherichia coli strain DH5α, used for general DNA manipulation, was routinely cultivated at 37°C in Luria-Bertani (LB) medium ( 19 ) and supplemented with 50 μg/ml ampicillin when needed.

Techniques: Plasmid Preparation, Clone Assay, Marker, Expressing